344 PRACTICAL PHYSIOLOGY 



VI. Uric Acid. Apply murexide test. 



VII. Blood Pigment. (1) Examine by means of the spectroscope. 

 A, the original fluid ; B, the same after reduction ; C, the same 

 after the addition of caustic alkali and heating. By this latter method 

 alkali hsematin is formed. This itself does not give a vfery distinct 

 absorption band, but if a reducing agent (NH 4 HS) be added to it 

 haemochromogen is formed, which has two very distinctly marked 

 bands in about the same position as those of oxyhsemoglobin. 



(2) Apply the guaiac and ozonic ether test. 



When it is desired to ascertain whether Ferments be present it is 

 necessary to add a piece of coagulated egg-white, or of washed fibrin, 

 to the original fluid, and to place the mixture on a waterbath heated 

 to body temperature. If, after an hour, the digest gives a distinct 

 proteose reaction, and this was not obtained in the original fluid, the 

 presence of a proteolytic ferment may be assumed ; pepsin, if the 

 original fluid react acid, and trypsin, if it react alkaline. If proteoses 

 are present in the fluid itself, some method like Mett's must be 

 employed to identify the ferment. 



For the detection of Diastatic and Lipolytic ferments, the methods 

 described in the text must be employed. 



For the detection of the various substances which may occur in the 

 urine, the test and reactions described in the text must be applied . 



NITROGEN FACTORS FOR CERTAIN ORDINARY METABOLIC PRODUCTS 

 FOUND IN URINE. 



Urea = N x 2'145 



Ammonia = N x 1'214 



Uric Acid = N x 3' 00 



Great inine = N x 2 '69 5 



Creatine = N x 3' 12 



