PROTOPLASM 



^ V 



A.. 



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% . v . "l 



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FIG. 13. 



a, b, c, and d, forms of precipitate in 5 per cent, albumose, dissolved in 0"2 per cent, potassium hydroxide, when 

 acted on by different reagents. Magnified about 600 diam. 



a, with 1 per cent, platinum chloride. 

 6, with Flemming's solution. 



c, with O'B per cent, chromic acid. 



d, with Altmann's mixture of potassium bichromate and osmic acid. 



f, 20 per cent, albumose, faintly acid, precipitated by mercuric chloride, stained with iron haematoxylin and 

 then differentiated. Note fusion of globules to form aggregates (a and b). 



(After Alfred Fischer. ) 



FIG. 14. DIFFERENTIAL STAINING OF GRANULES CONSISTING OF 

 SAME SUBSTANCE, ACCORDING TO THEIR DIMENSIONS. 



40 per cent, albumose, acted on first by 2 '5 per cent, potassium bichromate, which caused 

 turbidity, merely, in the solution. Subsequent acidification with acetic acid caused 

 precipitation of granules of great variety in size. In a a preparation of this mixture 

 was stained by Flemming's method in inverse order of dyes, namely, gentian violet, 

 acid alcohol, safranin. b was stained in the usual order with safranin, acid alcohol, 

 gentian violet. The darker granules in the figures must be supposed to be of a 

 violet colour ; the paler ones, red. In a the small granules are red ; the larger ones 

 violet. In b the opposite is the case. Staining with different d.\es has, thus, no 

 necessary relation to difference of chemical nature. 



(After Alfred Fischer.) 



