XX LABORATORY AND PERMANENT OUTFIT. 



but a good hand-lens, properly mounted, will answer the 

 same purpose. See Arthur, Barnes, and Coulter, Plant 

 Dissection, p. 2. 



6. Glassware and miscellaneous articles. A stock of 

 common plates and bowls, beakers, glass tubing, bell-jars, 

 test-tubes, metric rules, etc., will be required, but are best 

 purchased as needed, at the discretion of the teacher. 



REAGENTS. 



Of the reagents most employed in botanical work the 

 following are required : 1 - 



7. Alcohol. For preserving plant-tissues, except in 

 cases involving the most delicate operations, three grades 

 of alcohol are all that will be needed. The lowest grade 

 (between 45 and per cent) is composed of equal parts 

 of alcohol of commerce and distilled water. The inter- 

 mediate grade (between 70 and 75 per cent) is prepared 

 by adding 25 parts of distilled water to 75 parts of 

 commercial alcohol. The highest grade is the alcohol of 

 commerce (approximately 95 per cent). 



Parts of plants to be preserved are allowed to remain 

 24 hours in the lowest grade of alcohol, then for the same 

 length of time in alcohol of intermediate strength, and 

 finally are placed in 95 per cent alcohol, in which they 

 may be kept indefinitely. It is necessary to guard against 

 attempting to preserve too much material in a given 

 quantity of alcohol, as decomposition is likely to take 

 place. 



1 Reference may be made to various works in which reagents and 

 methods are discussed at much greater length. Among these are Stras- 

 burger and Hillhouse, Practical Botany ; Behrens, Guide to the Use of 

 the Microscope in Botany ; Zimmermann, Die botanische Mikrotechnik. 



