368 CHYLE, LYMPH, TRANSUDATES AND EXUDATES. 



nucleate. We cannot say whether this applies to the two nucleohistones 

 (if there are two). The nucleohistone or mixture of nucleohistones 

 behave like an acid, whose salts, especially the calcium salt, have been 

 closely studied by HUISKAMP. On the electrolysis of a solution of alkali 

 nucleohistone in water HUISKAMP also found that the nucleohistone 

 collected in traces at the anode, and that the sodium compound is there- 

 fore ionized in the solution. The nucleic acid-calcium histone-com- 

 pound has been prepared, it seems, in a pure state by BANG, and he found 

 the following average composition: C 43.69; H 5.60; N 16.87; S 0.47; 

 P 5.23; Ca 1.71 per cent. 



The nucleohistone prepared by HUISKAMP'S method of precipitating with 

 CaCU is, according to him, a mixture of two nucleohistones, of which one, the 

 a-nucleohistone, contains 4.5 per cent phosphorus, and the other, /3-nucleohistone 

 contains, on the contrary, only in round numbers 3 per cent phosphorus. 1 As 

 the two nucleohistones are poorer in phosphorus than the nucleic acid-histone 

 compound analyzed by BANG, and as HUISKAMP on cleavage of his preparation 

 did not, like BANG and MALENGREAU, obtain pure nucleic acid, it is still a ques- 

 tion whether HUISKAMP was working with sufficiently pure substances. 



In regard to the methods used by the above investigators in the 

 isolation of the bodies in question we must refer to the original publications. 



In connection with the so-called nucleohistone, attention must be called to 

 tissue fibrinogen and cell fibrinogen, which are compound proteins, and are claimed 

 by certain investigators to stand in close relation to the coagulation of the blood. 

 These may be in part nucleoproteins and in part also nucleohistones. To this same 

 group belong also the important cell constituents described by ALEX. SCHMIDT 2 

 and called cytoglobin and preglobulin. The cytoglobin, which is soluble in water, 

 may be considered as the alkali compound of preglobulin. 'The residue of the 

 cells left after complete extraction with alcohol, water, and salt solution has 

 been called cytin by ALEX. SCHMIDT. 



Besides the above-mentioned and the ordinary bodies belonging to 

 the connective-tissue group, small quantities of fat, leudne, sucdnic 

 add, lactic add, sugar, and traces of iodothyrin are present. According 

 to GAUTIER 3 arsenic also occurs in very small amounts, and no doubt 

 here as well as in other organs it is related to the nuclein substances. The 

 richness in nuclein bodies explains the occurrence of large quantities 

 of purine bases, chiefly adenine, whose quantity, according to KOSSEL 

 and ScHiNDLER, 4 is 1.79 p. m. in the fresh organ and 19.19 p. m. in the 

 dry substance, and guanine. The bodies thymine and (uradlf) obtained, 

 besides lysine and ammonia, by KUTSCHER, as products of autodiges- 

 tion of the gland, probably have a similar origin. Among the enzymes, 



1 Zeitschr. f. physiol. Chem., 39. 



2 See footnote 1, p. 307. 



3 Compt. Rend., 129. 



4 Zeitschr. f. physiol. Chem., 13; Kutscher, Zeitschr. f. physiol. Chem., 34. 



