EXPERIMENTAL. 



hydrolysis to 257.8 grams of water and ash-free leucosin. This was hydro- 

 lyzed and the glutaminic acid separated as hydrochloride. When dried in 

 vacuo over sulphuric acid, 12.39 grams of the hydrochloride were obtained, 

 which melted at 198 with effervescence. 



Nitrogen : 0.5482 gram substance gave NH 3 = 4.i7 cc. HC1 (i cc. HC1 = o.oi gram N). 



Chlorine : 0.2528 gram substance gave 0.1989 gram AgCl. 



Calculated for C 5 H 10 O 4 NC1, N 7.64, Cl 19.35 p. ct; found, N 7.61, Cl 19.45 p. ct. 



The filtrate from the glutaminic acid hydrochloride was concentrated to a 

 sirup under reduced pressure, the residue taken up in alcohol and saturated 

 with dry hydrochloric acid gas. The solution was then evaporated to a 

 thick sirup under reduced pressure, the residue again esterified with alcohol 

 and hydrochloric acid, and the solution concentrated as before. The esteri- 

 fication was again repeated, the final concentration being made at a pressure 

 of 10 mm. from a bath, the temperature of which did not rise above 40. 

 The free esters of the amino-acids were then liberated from the residue, 

 extracted with ether, and dried with potassium carbonate and anhydrous 

 sodium sulphate in the usual way. The aqueous layer was then made 

 strongly acid with hydrochloric acid and the salts removed by concentra- 

 tion and treatment with alcoholic hydrochloric acid. The alcoholic extracts 

 containing the hydrochlorides of the amino-acids were evaporated to a thick 

 sirup under reduced pressure and the residue esterified as above described. 

 The free esters were then liberated and their ether solution dried as before. 

 After distilling off the ether on the water-bath, at atmospheric pressure, the 

 residue was distilled with the following results : 



| Fraction I. Temperature of bath up to 75. ) 

 (Pressure, 12 mm. Weight, 21.76 grams. j 



This fraction was saponified directly after collection by evaporating on the 

 water- bath with concentrated hydrochloric acid. The residue was esterified 

 with alcohol and hydrochloric acid and allowed to stand for several days 

 on ice. The glycocoll ester hydrochloride which separated weighed 1.73 

 grams and melted at 145. 



