3 2 THE PROTEINS OF THE WHEAT 



Nitrogen: 0.2851 gram substance, dried in vacuo, gave NH 8 =2.88 cc. HC1 (i cc. 



HCl = o.oi gram N). 



Chlorine: 0.2843 gram substance, dried in vacuo, gave 0.2889 gram AgCl. 

 Calculated for C 4 H 10 O 2 NCI, N 10.05, Cl 25.40 p. ct.; found, N 10.12, Cl 25.12 p. ct. 



In the filtrate from the glycocoll ester hydrochloride the free amino-acids 

 were regenerated and subjected to fractional crystallization in water and 

 alcohol. There were obtained 3.06 grams of nearly pure alanine. 



Carbon and hydrogen : 0.3682 gram substance gave 0.5460 gram CO 2 and 0.2649 



gram H 2 O. 



Nitrogen : 0.2508 gram substance gave NH 3 = 3.92 cc. HC1 (i cc. HC1 = o.oi gram N). 

 Calculated for C 3 H 7 O 2 N, C 40.40, H 7.93, N 15.75 P- ct.; found, C 40.44, H 7.99, 



N 15.63 p. ct. 



The alanine decomposed at 290. 



f Fraction II. Temperature of bath up to 80. ) 

 {Pressure, 10 mm. Weight, 25.86 grams. } 



After saponifying with boiling water, the solution of the amino-acids was 

 evaporated to dryness under reduced pressure. The dried residue, which 

 weighed 18.78 grams, was extracted with boiling alcohol, whereby 1.68 

 grams went into solution. From the part insoluble in alcohol there were 

 isolated, by fractional crystallization, 5.53 grams of leucine, 5.79 grams 

 alanine, and 0.47 gram of substance which had the percentage composition 

 of amino-valerianic acid. 



Carbon and hydrogen: 0.2094 gram substance gave 0.3913 gram CO 2 and 0.1793 



gram H 2 O. 

 Calculated for C 5 H U O 2 N, C 51.22, H 9.48 p. ct.; found, C 50.96, H 9.51 p. ct. 



From the more soluble part of this fraction glycocoll was isolated as the 

 hydrochloride of the ester. This weighed 2.78 grams, equivalent to 1.50 

 grams of glycocoll, and melted at 144 to 145. 



f Fraction III. Temperature of bath up to 125. ) 

 ( Pressure, 0.8 mm. Weight, 49.6 grams. j 



Fraction III was saponified by boiling with 10 parts of water for 10 hours, 

 and the solution evaporated to dryness under reduced pressure. After 

 extracting the proline with boiling alcohol the insoluble part was fractionally 

 crystallized. There were obtained 23.72 grams of leucine and 2.62 grams 

 of alanine. The isolated leucine decomposed at 298. 



Carbon and hydrogen: 0.1835 gram substance dried at 110 gave 0.3670 gram CO 2 and 



o. 1670 gram H 2 O. 



Nitrogen : 0.3086 gram substance gaveNH, = 3.34cc. HC1 (i cc. HCl = o.oi gram N). 

 Calculated for C 6 H 13 O 2 N, C 54.89, H 10.01, N 10.70 p. ct.; found C 54.63, H io.ii, 



N 10.82 p. ct. 



