EXPERIMENTAL. 



43 



These analyses, when calculated free from nucleic acid and ash, as was 

 done for the albumin preparations, in the manner described on page 27, gave 

 the results set out in table 8. 



. Composition of the globulin contained in the preparations extracted 

 from the wheat embryo by sodium-chloride solution. 



These figures plainly indicate that these globulin preparations are mixtures 

 of nucleates of protein substance of the same ultimate composition, and con- 

 tain from 5 to 15 per cent of nucleic acid. This protein has very nearly the 

 same composition as the globulin occurring in the kernel of wheat, rye, 

 barley, and maize. In the entire kernel so little of this globulin is present 

 that it is difficult to prepare it pure therefrom. For this reason the analyses 

 given in table 9 do not agree as closely as they might otherwise be expected 

 to. From the whole seed this globulin is obtained entirely free from phos- 

 phorus, which is probably due to the much greater proportion of protein 

 matter to nucleic acid in the entire seed compared with that existing in the 

 embryo. 



TABLE 9. Composition of the globulin contained in various cereals. 



1 Osborne, Journal American Chemical Society, 1895, xvn, p. 429. 



2 Chittenden & Osborne, American Chemical Journal, 1891, xiu, pp. 327, 385, 



and 1892, xiv, p. 20. 



3 Osborne, Journal American Chemical Society, 1895, xvn, p. 539. 



