EXPERIMENTAL. 47 



with sodium chloride, which produced a small precipitate. This was filtered 

 out, dissolved, and its solution dialyzed in water. The salt-saturated filtrate 

 was likewise dialyzed, and when both solutions were free from chlorine the 

 dialyzers were transferred to alcohol and the proteose thereby precipitated. 

 The proteose separating on saturation with salt gave 0.6 gram of prepara- 

 tion 43 ; that from the salt-saturated solution 0.97 gram of preparation 44. 

 This small yield of proteose indicates that the greater part had diffused 

 through the parchment paper during the long dialysis to which the solutions 

 had been subjected. 



Another crude product was obtained by dialyzing an aqueous extract in 

 alcohol after separating the leucosin which had been coagulated by heat. 

 This, weighing 35 grams, was dissolved in water and the insoluble matter 

 filtered out, washed and dried, giving preparation 45, weighing 7.26 grams. 



The filtered solution was saturated with ammonium sulphate, the pre- 

 cipitate dissolved in water, and the clear solution dialyzed in distilled water 

 until free from sulphates, and then in alcohol. The substance thus sep- 

 arated was again dissolved in water and its solution saturated with salt ; the 

 precipitate thus produced was dissolved in water, and its solution, as well as 

 the salt-saturated filtrate, were dialyzed in water. When free from chlorine, 

 these solutions were dialyzed in alcohol and yielded, respectively, prepara- 

 tions 46, weighing 4 grams, and 47, weighing 1.84 grams. 



Another preparation of crude proteose was obtained by extracting the 

 meal as described on page 41 with 3 per cent sodium-chloride solution heated 

 to 70, dialyzing the extract in water, coagulating the leucosin by heat, and 

 precipitating the proteose by dialysis in alcohol. A mixture of such prep- 

 arations, weighing 31.6 grams, was treated with water, the insoluble matter 

 filtered out, washed, and dried, giving 5.16 grams of preparation 48. 



The filtered solution was saturated with ammonium sulphate, the precipi- 

 tate dissolved in water, the solution dialyzed in distilled water till free from 

 sulphate, and then in alcohol. The separated proteose was redissolved in 

 water and its solution saturated with sodium chloride. The precipitate 

 which resulted was filtered out, dissolved in water, and its solution, as well 

 as the salt-saturated filtrate, were dialyzed in v water till free from chlorine, 

 and finally in alcohol. 



The products thus obtained formed, respectively, preparation 49, weigh- 

 ing 0.75 gram, and 50, weighing 1.35 grams. One other proteose prepara- 

 tion was made from the aqueous extract described on page 26, from which 

 the phosphorus was largely separated by making it slightly alkaline and 

 adding calcium chloride. After heating the extract to boiling and filtering 

 out the coagulum, the filtrate was dialyzed in alcohol, the resulting pre- 

 cipitate dehydrated with absolute alcohol, dried over sulphuric acid, redis- 

 solved in water, and precipitated by saturating with ammonium sulphate. 



