74 THE PROTEINS OF THE WHEAT KERNEL. 



The chlorine was removed from the solution used for determining the 

 specific rotation and the substance racemized by heating with 20 cc. of 

 water and 7 grams of crystallized barium hydroxide for 19 hours in an 

 autoclave at 175. The barium was quantitatively removed with sulphuric 

 acid, and the a-naphthyl-hydantoic acid prepared according to the directions 

 of Neuberg & Manasse. 1 This crystallized in long needles and melted 

 constantly on repeated recrystallization from 40 per cent alcohol at 180 

 to 181. 



Carbon and hydrogen: 0.3235 gram substance, dried at 90, gave 0.7936 gram CO 2 and 



o. 1909 gram H 2 O. 

 Calculated for C 16 H 18 O 3 N 2 , C 67.07, H 6.35 p. ct.; found, C 66.90, H 6.56 p. ct. 



By racemizing the remaining mixture of undetermined amino-acids we 

 were unable to isolate any more amino-valerianic acid. 



(IV) 8.6 grams alanine. This was racemized by heating with an excess 

 of barium hydroxide and coupled with -naphthyliso-cyanate according to the 

 directions of Neuberg & Manasse. The hydantoic acid, which crystal- 

 lized in prisms, melted at 197. 



Carbon and hydrogen: 0.3146 gram substance, dried at no , gave 0.7480 gram CO 2 and 



o. 1560 gram H 2 O. 

 Calculated for C U H U O 3 N 2 , C 65.06, H 5.48 p. ct.; found, C 64.84, H 5.51 p. ct. 



f Fraction III. Temperature of bath, up to 120. 

 | Pressure, 0.8 mm. Weight, 127.38 grams. 



This fraction was boiled for 5^ hours with 8 parts of water. The solution, 

 evaporated to dryness under reduced pressure, gave 98 grams of amino-acids 

 or 80 per cent of the esters. Of this 59.94 grams were soluble in alcohol. 

 From the part insoluble in alcohol, by systematic fractional crystallization, 

 there were isolated 33.06 grams of leucine and 4.57 grams of alanine. The 

 leucine decomposed at about 298. 



Carbon and hydrogen: 0.2416 gram substance, dried at 110, gave 0.4872 gram CO 2 



and 0.2166 gram H 9 .O. 



Nitrogen : 0.2690 gram substance gave NH 3 = 2.85 cc. HC1 (i cc. HC1 = o.oi gram N). 

 Calculated for C 6 H 13 O 2 N, C 54.89, H 10.01, N 10.70 p. ct ; found, C 54.99, H 9.96, 



N 10.59 P- ct * 



The alcohol- soluble substance from fraction II was united with that from 

 fraction III. The solution was evaporated to dryness under reduced press- 

 ure, and the residue taken up in water and boiled gently for about an hour 



1 Neuberg & Manasse, Berichte der deutschen chemischen Gesellschaft, 1905, xxxvm> 

 P- 2359- 



