7 6 



THE; PROTEINS OF THK WHEAT KERNEL. 



The total crystalline racemic copper salt was equal to 41.96 grams of 

 r-a-proline. 



From the other half of /-proline copper salt the free proline was regen- 

 erated and recrystallized from alcohol. A small part only was obtained in 

 a crystalline condition, which melted at 205 to 206. 



Carbon and hydrogen : 0.2729 gram substance, dried over H 2 SO 4 , gave 0.5198 gram 



CO 2 and o. 1977 gram H 2 O. 



Nitrogen : 0.2453 gram substance gave NH 3 = 2.95 cc. HC1 (r cc. HC1 = o.oi gram N). 

 Calculated for C 5 H 9 O 2 N, C 52.12, H 7.90, N 12.20 p. ct. ; found, C 51.95, H 8 04, 



N. 12.03 p. ct. 



From fraction III there were isolated 4.57 grams alanine, 33.06 grams 

 leucine, and 70.49 grams -proline, including in this last that from frac- 

 tion II, which was not weighed separately. 



This was treated with water and shaken out with ether according to the 

 procedure described by Bmil Fischer. 1 



The ether was carefully removed by evaporation and the residual phenyl- 

 alanine ester saponified by dissolving in concentrated hydrochloric acid 

 and evaporating on a water-bath. The phenylalanine hydrochloride weighed 

 29.14 grams, equivalent to 23.87 grams of free phenylalanine. The phenyl- 

 alanine hydrochloride was recrystallized from strong hydrochloric acid. 

 It was decomposed by evaporating with an excess of ammonia and the 

 phenylalanine recrystallized from water. It melted, on slow heating, at 

 263 to 265 . 2 



Carbon and hydrogen : 0.3051 gram substance, dried at 110, gave 0.7322 gram CO 2 and 



0.1792 gram H 2 O. 



Nitrogen : 0.3020 gram substance gave NH 3 = 2.53 cc. HC1 (i cc. HC1 = o.oi gram N). 

 Calculated for C 9 H U O 3 N, C 65.40, H 6.73, N 8.50 p. ct. ; found, C 65.44, H 6.53, N 8.38 



p. ct. 



Fischer, E., Zeitschrift fur physiologische Chemie, 1902, xxxvi, p. 274. 

 'Fischer, Kmil, & Abderhalden, Zeitschrift fur physiologische Chemie, 1902, xxxvi, 

 p. 268 ; Erlenmeyer & lyipp, Annalen der Chemie, 1883, ccxix, p. 197. 



