78 THE: PROTEINS 6? THE WHEAT KERNEL. 



Specific rotation. Dissolved in 20 per cent hydrochloric acid, (a) -=- = 



-f 31-47. 



Fischer & Dorpinghaus found +31.91 for a preparation from horn, 

 4-30.45 from gelatin, and +28.20 for one from casein. 1 



The residue which remained after removing the esters with ether from 

 the original solution of the products of hydrolysis was treated in the 

 way described by Kmil Fischer 2 for the isolation of oxy-proline. The 

 only substance, however, that could be isolated was serine, of which 

 0.87 gram was obtained, which browned at about 219 and decomposed at 

 about 240. 



Carbon and hydrogen: 0.2987 gram substance, dried at 110, gave 0.3743 gram CO 2 



and 0.1844 gram H 2 O. 

 Calculated for C 3 H 7 O 3 N, C 34.24, H 6.73 p. ct. ; found, C 34.18, H 6.85 p. ct. 



In the filtrate from this serine /3-naphthalene-sulphone chloride failed to 

 give any definite product. 



There were thus isolated in the two distillations from fraction I, 6.68 

 grams ; from fraction II, 8. 59 grams, and from fraction III, 4.57 grams of 

 alanine, or 19.84 grams in all; from fraction II, 2.1 grams of amino-vale- 

 rianic acid ; from fraction I, 0.86 gram ; from fraction II, 22.10 grams ; from 

 fraction III, 33.06 grams of leucine in all 56.02 grams ; from fractions II 

 and III, 70.49 grams of proline ; from fraction IV, 23.47 grams of phenyl- 

 alanine, 5.76 grams of aspartic acid, and 0.42 grams of serine, and from the 

 residue which remained after extracting the esters 0.87 gram of serine, or 

 1.29 grams in all. 



From the main solution of the total products of hydrolysis there were 

 obtained 300 grams of glutaminic acid, and from the residues after dis- 

 tilling the esters 59.5 grams, or in all 359.5 grams. 



As the amount of proline found in this hydrolysis was so great, the result 

 was confirmed by a second hydrolysis, and another effort made to obtain 

 glycocoll, which, if present in very small amount, might have escaped 

 detection. 



For this purpose 500 grams of gliadin, air^dry, equal to 439.6 grams dried 

 at no , were hydrolyzed in the same way as in the preceding hydrolysis. 

 After esterifying and shaking out the esters three times, as before, the ether 

 was removed by distillation at 760 mm. and the esters distilled. 



a Cf. Fischer, Bmil, & Dorpinghaus, Zeitschrift fiir physiologische Chemie, 1902, 

 xxxyi, p. 475. 

 2 Fischer, Kmil, Berichte der deutschen chemischen Gesellschaft, 1902, xxxv, p. 2660 



