274 BACTERIAL POISONS. 



The filtrate was poisonous, and produced, in frogs, paral- 

 ysis and stoppage of the heart in diastole Addition of 

 platinum chloride and alcohol precipitated two bases. One 

 of these, although possessing a curara-like action, did not 

 affect the heart. When its solution was heated for twenty- 

 four hours on the water-bath, it caused general paralysis 

 and stoppage of the heart. The platinochloride contained 

 38.05 per cent, of platinum. 



The other base also possessed a slight curara-like action, 

 and its platinochloride gave, on analysis, 40.92 and 39.4 

 per cent, of platinum. 



BRIEGER found a basic substance in small quantities in 

 cultures of the staphylococcus pyogenes aureus on bouillon 

 and beef-broth (II., 74). The hydrochloride formed groups 

 of colorless, non-deliquescent needles. With platinum 

 chloride it yielded a double salt, crystallizing in needles, 

 and containing 32.93 per cent, of Pt. For its reactions, 

 see Table I. 



From aqueous as well as alcoholic solutions of cultures of 

 staphylococcus aureus LEBP:R (1888) isolated a crystalline 

 substance which he named phlogosine. The composition 

 of this substance is not known. It does not seem to con- 

 tain nitrogen, and inasmuch as it blackens silver it prob- 

 ably contains sulphur. It crystallizes in fine needles which 

 are soluble in ether and in alcohol ; difficultly soluble in 

 water. It sublimes in needles. Alkalies precipitate it as 

 amorphous yellow floccules which are soluble in acid and 

 then can be recrystallized. With potassium ferricyanide 

 and ferric chloride it yields a blue color, and with potassio- 

 mercuric, cadmic, and bismuth iodides precipitates which 

 are soluble in excess. No precipitate is produced by gold 

 or platinum chlorides, phosphotungstic or molybdic, tan- 

 n ic or picric acids. 



A small quantity applied to the conjunctiva produces 

 intense inflammation, suppuration, and necrosis. Intro- 

 duced into the anterior chamber it induces intense suppura- 

 tion and keratitis. The substance is entirely distinct from 

 the base obtained by BRIEGER, described above. 



