WATER ANALYSIS 21 



Free and saline ammonia. 

 Albuminoid ammonia. 

 Oxygen absorption. 

 Nitrates. 

 Nitrites. 



Bacteriological Examination. 



(a) Absolute minimum. 



1. Enumeration of bacteria growing in a medium at air 

 temperature i8-22 C. 



2. Search for Bacillus coli. If found identification 

 and enumeration. 



(b) Additional. 



3. Enumeration of bacteria growing at blood heat 37 C. 



4. Search for and enumeration of streptococci. 



5. Search for Bacillus enteritidis sporogenes. 



(c) Special procedures. 



6. Isolation of Bacillus typhosus from water. 



7. Isolation of Spirillum cholerae. 



COLLECTION OF SAMPLE. 



A fair average sample should be taken in a clean glass 

 vessel with a glass stopper. In filling the vessel from a 

 pond, lake, reservoir, or river, the mouth of it should be 

 sunk two inches below the surface, and the vessel should 

 be filled and emptied once or twice. If a surface specimen 

 is wanted, then of course the sample will be so taken. 

 When sampling water from a pipe or tap, unless the effect 

 of the water on the pipes is under examination, the water 

 should be allowed to run to waste for a few minutes before 

 filling the vessel. 



The stopper should be tied in but not sealed. 



A convenient receiver is a Winchester quart bottle 

 which holds half a gallon, and this is a suitable quantity 

 for the usual analysis. 



Along with the sample, a written statement should be 

 sent, giving full particulars as to mode of collection, place, 

 time, recent meteorological conditions, reason why analysis 

 is desired, etc., etc. 



