IMMUNITY AND ANAPHYLAXIS 217 



with the antibody-producer or antigen. White-of-egg 

 being a viscous liquid is slowly absorbed from the peri- 

 toneum or subcutaneous spaces, and so the combination 

 of the antibody and antigen has not the suddenness or 

 instantaneousness which follows intravenous injection. 

 (This deduction suggests that the antigen may act in a 

 catalytic manner, causing an amount of chemical action 

 out of proportion to its own mass injected. In such a 

 case, the symptoms may be partly due to the accom- 

 paniments of rapid chemical action, such as the evolution 

 of heat, etc.) 



Antianaphylaxis can be produced to white-of-egg, as 

 for serum, by the method of injecting small doses during 

 the " incubation " time of anaphylaxis ; or by the injection 

 of a minute dose, followed by a larger dose in 10 minutes, 

 and so on for four doses. A guinea-pig, previously passively 

 sensibilized, had o^Vo- c.c. injected intravenously ; in 

 10 min. another injection of ^^ c.c. ; in another 10 

 minutes, a third injection, this time of -fa c.c. ; and finally 

 one of i c.c. Thereafter the injection of 2 c.c. of white-of- 

 egg, non-diluted, caused uneasiness but nothing further. 

 Antianaphylaxis can also be established by oral and by 

 rectal feeding, in two to four days. The state of anti- 

 anaphylaxis is not so lasting as in the case of serum ; it 

 lasts about three weeks, and two weeks where obtained 

 by the oral or rectal method. 



Besredka and Bronfenbrenner also found that the 

 reactions were strictly specific, and that the anaphylaxis 

 produced by white-of-egg was in the main specific. Feeble 

 reactions were given by the white-of-egg of pigeon and 

 turtle-dove. The anti-anaphylactic is strictly specific. 

 Similar experiments were made with heated white-of-egg, 

 with like results. The one protects little against the other, 

 so that they conclude that their chemical constitution is 

 different. 



III. SERUM-GLOBULIN. 



Turro and Gonzales have investigated the subject of 

 anaphylaxis to determine the nature of the substance 

 which causes anaphylaxis with blood serum. The 

 globulins were precipitated from horse's serum, and the 



