218 PUBLIC HEALTH BACTERIOLOGY 



residual serum was kept. Guinea-pigs were injected with 

 i c.c. of a 0-33 per cent solution of the globulins ; and 

 12 days after, it was found that the minimal test dose was 

 i c.c. of a 0-66 per cent solution (= 2 c.c. of the former 

 solution). Anaphylactic shock developed rapidly, and there 

 was a rapidly ascending paralysis, beginning in the hind 

 limbs and causing death through asphyxia when the 

 respiratory centres became affected, in 2 to 4 minutes. 

 There were no convulsions, but in the male animals there 

 was an abundant emission of serum. 



Another set of fresh guinea-pigs were given i c.c. of a 

 i per cent dilution of the globulin-free serum ; and in 

 twelve days thereafter, a test dose of the globulin solution 

 produced no symptoms of anaphylaxis. (No mention is 

 made of a test with the globulin-free serum itself.) An 

 animal injected with normal serum, and later with trie 

 globulin solution test dose, showed intense muscular 

 tremors, (the animal jumping about), but all ended in 

 recovery. 



When the blood of a sensitized guinea-pig is mixed 

 with globulin solution, and i c.c. injected into the jugular 

 vein of a normal guinea-pig, the animal dies in 2 to 3 

 minutes with symptoms identical with those described 

 in animals sensitized with pure globulins. They proceeded 

 to study the anaphylactic poison, which they found is 

 readily destroyed by oxidation and light, is dialysable, 

 is thermostabile, and is soluble in alcohol and ether. They 

 conclude that it is alkaloidal in nature, and should be 

 considered a leucomaine, that is, a toxic substance produced 

 in the living body by proteid metabolism, and not by 

 bacterial action. 



IV. CLASSIFICATION. 



The foregoing resum& of the subject of anaphylaxis 

 shows that it can be classified in the same manner as 

 immunity into : 



1. Natural Anaphylaxis. 



2. Acquired Anaphylaxis. 



And each of these into sub-groups, thus : 



