NON-SPORING BACILLI 253 



succeeding day take the temperature at least three times. 

 In a healthy animal free from glanders, a local swelling, not 

 exceeding 3 inches in diameter, is produced at the seat 

 of inoculation, and a rise of temperature not exceeding 

 i C. (1-8 F.) ; and both swelling and temperature have 

 much subsided in twenty-four hours. In a horse suffering 

 from glanders, there appears within a few hours a firm, hot, 

 diffuse swelling, which reaches a maximum size in twenty- 

 four hours, is intensely tender during that time, and lasts 

 from three to nine days. The size of the swelling reaches at 

 least 5 inches in diameter. The temperature rises in six to 

 eight hours 1-5 to 2 C. (2-7 to 3-6 F.), reaching 104 to 

 1 06 F. The high temperature continues for eight to ten 

 hours (maximum about ten to sixteen hours after 

 injection), and then gradually falls, but is distinctly above 

 normal on the following day. This reaction is specific. 



3. Agglutination Test. The macroscopic or sedimentation 

 method in high dilutions (i-iooo) is preferred. Normal 

 horse serum may react in 1-500. 



Immunity. An attack of glanders does not confer 

 immunity. Artificial active immunization has been 

 attempted but has so far failed. 



Nodules. The nodules found in glanders show more 

 leucocytic infiltration and less proliferative change towards 

 formation of epitheloid ceUs than does tubercle. 



Prevention. The Glanders or Far Order of 1907, 

 issued by the Board of Agriculture, (i) Lays down com- 

 pulsory notification of actual or suspected disease ; 

 (2) Empowers local authority to slaughter at once any 

 diseased horse, ass, or mule ; (3) Enables local authority 

 to test suspected animals with mallein, and deal with 

 contacts. 



