STAINS 265 



EHRLICH'S ACID H^EMATOXYLIN. 



Water 100 c.c. 



Absolute alcohol 100 ,, 



Glycerin 100 



Glacial acetic acid 10 ,, 



Haematoxylin 2 grammes 



Alum in excess 



This mixture must be left in the light to ripen, which will 

 take some weeks or even months ; it gradually acquires a 

 dark red colour. 



Sections are stained in this fluid in a few minutes. 

 Material in bulk should remain in the stain from 2 hours to 

 2 or 3 days, afterwards washing in 70% alcohol. 



HEIDENHAIN'S IRON H^MATOXYLIN. 



Solution A 



Iron alum 4 grammes 



Distilled water 100 c.c. 



Solution B 



Hsematoxylin crystals -5 gram. 



Distilled water 100 c.c. 



Sections are placed in A for from half an hour to two hours, 

 then washed in tap water and transferred to B for half an hour. 

 They are then rinsed in tap water and again placed in A. 

 The stain is gradually washed out of the sections, and the 

 progress of differentiation should be carefully observed under 

 the microscope. When the stain has been washed out of all 

 parts of the section except the chromatin of the nuclei the 

 slide should be washed in running water for 10 minutes, 

 counterstained, dehydrated, and mounted. 



PICRO- ANILINE BLUE. 



To a saturated solution of picric acid in 92% alcohol add 

 aniline blue until the liquid becomes deep blue-green in colour. 



ANILINE BLUE. Counterstain to Borax-carmine. 



Aniline blue -2 gram. 



Alcohol (92%) 100 c.c. 



SAFRANIN. Counterstain for Hsematoxylin. 



Saturated solution in 92% alcohol. 

 This can be diluted with an equal quantity of water. 



