266 PHOTOMICROGRAPHY 



EOSIN. After Hsematoxylin (animal tissues). 



Eosin 1 gram. 



Alcohol (92%) 300 c.c. 



ORANGE G. After Heidenhain's Iron Haematoxylin and 

 sometimes after Ehrlich's Haematoxylin. 

 It is a precise plasma stain. 

 Saturated solution in water. 



IODINE GREEN. Counterstain to Carmalum, stains lignified 

 tissues in plant sections. 



Iodine green 1 gram. 



Alcohol (92%) 300 c.c. 



PICRIC ACID. After Borax-Carmine. 



Saturated solution in alcohol. 



As the picric acid dissolves out in alcohol it is sometimes 

 advisable to add it to the xylene used for final clearing before 

 mounting. 



Note. Industrial methylated spirit may be used in place 

 of 92% alcohol. 



STAINS FOR BACTERIA 

 CARBOL-FUCHSINE. 



Euchsine 1 part 



Absolute alcohol ' . . . . 10 parts 



5% aqueous solution of phenol (carbolic 



acid) 100 



LOFFLER'S METHYLENE BLUE. 



Caustic potash solution (1 in 10,000) 100 parts 



Saturated alcoholic solution of methylene 



blue 30 



GRAM'S METHOD. 



A. Saturated alcoholic solution of Gentian 



violet 11 parts 



Saturated aqueous solution of anilin oil . . 100 

 Mix and filter. 



B. Iodine 1 part. Potassium iodide 2 parts. Water 300 



parts. 



C. Saturated aqueous solution Bismark brown. 



