APPENDIX. 



LIST OF EEAGENTS. 



SALT SOLUTION, '75 per cent. 



Preparation. Dissolve 7'5 grins, common salt in 1 litre water. 

 Use. Living or fresh tissues are usually examined in it. 



GLYCERINE. Used either pure, or diluted with an equal bulk of 

 water. 



Use. For mounting microscopical objects. 

 ACETIC ACID, 1 per cent, solution. 



Preparation. Dissolve 10 c.c. glacial acetic acid in water : 



make up to 1 litre with water. 

 Use. For clearing tissues, and especially for rendering nuclei ^ 



more distinct. 



OSMIC ACID, 2 per cent, solution. 



Preparation. Dissolve 1 grm. osmic acid crystals in 50 c.c. 



distilled water. It must be kept in the dark. 

 Use. For killing microscopic animals, and for staining fats. 



Before use it is to be diluted with 1 to 3 times its bulk of 



water. 



CHROMIC ACID, 10 per cent, solution. 



Preparation. Dissolve 10 grms. chromic acid crystals in 



100 c.c. water. 

 Use. With or without addition of nitric acid for decalcifying 



bone, etc. As a hardening reagent it must be reduced to 



a 1 per cent, or '5 per cent, solution by addition of water 



before use. 



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