420 APPENDIX 



(b) Saturate 70 per cent, alcohol with alum. 



Mix 100 c.c. solution (a) with 800 c.c. solution (b) : 

 allow to stand a week. Decant and add a few drops 

 of saturated solution of hsematoxylin in absolute 

 alcohol. 



The solution should be of a deep violet-blue colour. 

 If it be reddish, add a solution of sodium carbonate 

 drop by drop till the violet-blue colour is obtained. 

 Use. Stain. The specimen must be previously placed in 

 strong alcohol. It will not give satisfactory results with 

 tissues which contain acid. 



OIL OF CLOVES. 



Use. Clearing reagent, i.e. it is used to remove alcohol from 

 an object before mounting it in Canada balsam. 



TURPENTINE. 



Use. Clearing reagent ; but chiefly used for dissolving paraffin 

 from sections before mounting them. 



CREASOTE AND TURPENTINE. 



Preparation. Mix 100 c.c. creasote and 400 c.c. turpentine. 



Allow to stand till clear. 



. Use. Same as turpentine, but must not be used for sections 

 cemented down with shellac. 



CANADA BALSAM. 



Preparation. May be used as bought from the chemist, or 

 diluted to a suitable consistency with benzol or turpentine. 

 Still better, evaporate to dryness : powder, and dissolve 

 in chloroform, benzol, or turpentine. 



Use. Mounting medium. Before being mounted in it, the 

 specimen must be dehydrated with absolute alcohol, and 

 cleared with oil of cloves or other clearing reagent. 



CARMINE INJECTION FLUID. 



Preparation. Bub up carmine in water, add ammonia drop 

 by drop, till the carmine is dissolved. Filter, evaporate 

 at a gentle heat, stirring constantly to get rid of the am- 

 monia. Eub up the residue in camphor-water. 



Use. For injecting small animals or portions of animals. It 

 must be weh 1 shaken before use. 



