AMOEBA 39 



NUCLEUS. Find in living specimen. Describe its appearance. 

 Does its form change? Does its position change? Is there more 

 than one nucleus? Notes required. 



Make a careful drawing 100 mm. in diameter, of the living 

 Amoeba under the high power, labeling all parts. Stipple one 

 pseudopodium to show structure of endoplasm and its inclusions 

 and in addition show structure of other important parts such as 

 nucleus and vacuoles which may not be included in this pseudo- 

 podium. In the remainder of the body indicate division between 

 ectoplasm and endoplasm by a dotted line. 



With the aid of ocular micrometer find the approximate size 

 of an Amoeba (see page 36, section 6). 



4. EXPERIMENTS. Notes required. 



(1) Object: to determine the effect of a mechanical stimu- 

 lus upon an Amoeba. 



Clamp slide firmly to stage and gently tap the cover glass 

 with a needle. Results? 



(2) Object: to determine the effect of increased tempera- 

 ture upon the activity of an Amoeba. 



Describe the effects of warming slightly upon movement and 

 upon the activity of contractile vacuoles. The slide may be 

 warmed slightly by clamping to the stage then bringing the tip 

 of the finger in contact with the bottom of the slide through the 

 open diaphragm. 



While completing the work on Amoeba be on the lookout for 

 specimens which are dividing and encysting. When found de- 

 scribe and draw. 



REFERENCES 



Dellinger, O. P. 1906. Locomotion of Amoeba and Allied Forms. 



Jour. Exp. Zool. 3 :337-35& 



Greenwood, M. 1886-87. On the Digestive Process in Some 

 Rhizopods. Part I. Jour. Physiology 7:253; Part II. Jour. 



Physiology 8:263. 



Schaeffer, A. A. 1920. Amoeboid movement. 156 pp. Princeton 

 University. 



