PARAMECIUM 41 



and Paramecium with reference to locomotion. Notes required. 



2. The body of a Paramecium consists of three distinct 

 parts; (a) a thin, non-living, external covering, or cell wall, the 

 pellicle, (b) a thicker, fixed layer, the ectoplasm, in which the 

 trlchocysts are embedded, and (c) the more fluid, granular, inner 

 mass, the endoplasm. 



3. To demonstrate the trichocysts run a little tannic acid 

 under the cover glass. This kills the animals upon contact, but in 

 so doing the trichocysts are shot out from the body. Describe 

 the effect the instant the tannic acid reaches an animal. Draw 

 (60 mm. long). Thoroughly wash off your slide and cover glass 

 and get a fresh preparation. Use high power on living speci- 

 mens and look for the trichocyst layer in the ectoplasm. Notes 

 required. 



4. To demonstrate the pellicle run a little fifteen percent, 

 alcohol under the cover glass. Notice how the pellicle becomes 

 separated from the ectoplasm. Explain the markings seen on 

 the pellicle with high power. Draw (60 mm. long.) Notes re- 

 quired. 



5. To observe the endoplasm and its movements get a fresh 

 preparation and before applying the coverglass add a litde car- 

 mine suspension to the water containing the Paramecia. The 

 carmine is added here for use in a later part of the work. Car- 

 mine particles, while of no food value, are taken into the body 

 along with food, thus by their color making the food vacuoles 

 very conspicuous. 



6. How does the endoplasm differ from the ectoplasm? Are 

 the two entirely distinct? Watch specimens crowd through nar- 

 row places. How are ectoplasm and endoplasm affected? Notes 

 required. 



/. Under high power watch the passage of carmine grains 

 down the gullet. Describe the formation into food vacuoles. 

 Where do they enter the endoplasm ? Do they move in a definite 

 direction? Compare motion with that of granules of endoplasm. 

 Notes required, 



