11 



PROTOZOA 



SPOROZOA. 

 GREGARINIDA. 



i. Free Monocystis from the sperm rosettes in the vesicula seminalis of the Earthworm. High 

 power. cor. cortical substance ; med. medullary granular substance ; nu. nucleus ; n, m. successive 

 changes of form of the Gregarine. 



N.B. Open the Earthworm from the dorsal side in salt solution. Remove alimentary canal 

 carefully and open median vesicula seminales. Note white threads streaming from the sperm rosettes 

 which are the Monocystis. Cut off small part of rosette with attached parasite and examine in 

 75 p.c. salt solution. 



2. Cysts from amongst developing spermatozoa in the lateral vesiculae seminales. (a) With two 

 unbroken individuals ; (b) the encysted individuals fused and more or less broken up ; (c) with young 

 lemon-shaped pseudonavicellae ; (d} with complete pseudonavicellse ; (e) enlarged drawing of a single 

 pseudonavicella seen under the highest available power, es. spore-case. 



