222 CLINICAL DIAGNOSTICS. 



where tubercular processes in this organ seem to be absent. 

 Microscopical examination of milk for tubercle bacilli is very 

 difficult and the results unreliable, therefore we resort to 

 intraperitoneal inoculation of Guinea pigs with the fresh milk 

 of a suspected animal. If tubercle bacilli are contained in 

 the milk tubercular nodules will develop on the peritoneum 

 (omentum), spleen and liver in the course of two weeks. If 

 the Guinea pigs do not die before, they are killed at the end 

 •of six weeks and carefully examined for tuberculosis. 



II. Glanders, 



In view of the great infectiousness and incurability of 

 glanders, the object of the veterinarian is to determine the 

 presence or absence of this disease at the earliest possible 

 date. However, horses affected with glanders show no symp- 

 toms or at least no characteristic symptoms in the early stages 

 of the disease ; for this reason horses that have been 

 exposed to an infection with glanders 

 are subjected, to a mallein test, with the 

 object of thus enabling us to recognize the disease. If the 

 animals show symptoms of the disease we endeavor to obtain 

 some of the pathological products or secretions and with them 

 inoculate experimental animals which are known from experi- 

 ence to be susceptible to the disease and develop it in a char- 

 acteristic form. 



Mallein inoculation. ^lallein is the toxin of the bacilli 

 of glanders and is obtained from their cult-ures in a manner 

 analogous to that employed for obtaining tuberculin. The 

 crude preparation is a fluid, obtainable from the manufacturer 

 and injected in doses designated. It may also be obtained in 

 the dry or powdered form and is thus injected in doses of 0.02 

 — 0.1 G. according to the weight of the animal. It is best 

 to have the solution of the dry tuberculin prepared by the 

 manufacturer. 



