DIAGNOSTIC INOCULATION. 223 



Technique. This is the same as for tuberculin inocu- 

 lation. Taking temperature of animal to be tested, two or 

 three times at definite intervals before inoculation ; inoculation 

 between 10-12 P. M., and taking temperatures again on next 

 day beginning at 5 A. M., and repeating every two hours until 

 6 P. M. 



Interpretation of Results. The International Veterin- 

 ary Congress has accepted the following principles for guid- 

 ance in interpreting the results of a mallein test : 



1. A positive reaction to mallein confirms the diagno- 

 sis of glanders only when it^possesses a typical character. 



2. A typical reaction consists of an elevation of temper- 

 ature of at least two degrees centigrade [3.8° F.] and must 

 exceed 40° C. [104° F.] The temperature curve usually re- 

 mains at an elevation for some time or it may make a slight 

 drop and rise again on the same day. On the second, and 

 sometimes on the third day. there will be more or less eleva- 

 tion of temperature. A local as well as a general or con- 

 stitutional reaction is also observed. 



3. Elevations of temperature less than 40° C. [104° F.], 

 as well as greater elevations of an atypical character require 

 re-testing of the animal. 



. 4. Gradually attained high temperatures of considerable 

 duration, eVen if not typical, indicate the existence of glanders. 



5. A local typical infiltration ol the tissues at the point 

 of inoculation is a positive indication of the existence of 

 glanders, even in cases where no thermal or general organic 

 reaction occurs. 



6. All malleinized animals, whether they react or not, 

 must be subjected to the mallein test twice, at intervals of ten 

 to twenty days. 



The results of the mallein test cannot be compared with 

 those of the tuberculin test ; they are less reliable. There is 

 no doubt that the varying results obtained from the use of 



