Cellular Content of Milk. 



29 



ignated up to the present as "Nissen's Globules." According to 

 Ottolenghi they are derived either from leucocytes or from 

 epithelia. 



If fat-containing cells break down in this manner, fat globules 

 in the shape of grape-like bunches, and single fat globules result, 

 which are united by a mesh of fine protoplasm, or they are sur- 

 rounded in the form of a moon by a narrow border of protoplasm, 

 which crowded to one side rests like a cap on the fat globules. 

 Such moon and cap formations may also result in another way. 

 The leucocytes (mac- 

 rocytes), crowd on to 

 the dead or dying cells, 

 eat their way into the 

 cell bodies and establish 

 in the more and more 

 distending cell actual 



Fig. 14. 



* * 



*n 



* 









* 



* >'; 



** 



* 



V . 



C.' 



lacunae, in which the de- 

 vouring leucocytes lie. 

 The remains of the pro- 

 toplasm and of the cell 

 and nuclear membranes 

 float in the shape of 

 caps and moons in the 

 milk protoplasm until 

 the swelling or further 

 breaking down converts 

 them into spheres or 

 globules. At the same 

 time of course the mac- 

 rocytes may them- 

 selves degenerate in the 

 cell, and no longer pre- 

 sent a recognizable nu- 

 cleus. In such cases its 

 respective lacuna con- 

 tains homogeneous, 

 sharply circumscribed 

 proteid globules. 



The author considers these epithelial cells which have been destroyed by macro- 

 phages, as identical with the albuminophores of Bab and Schulz which they described 

 as large lymphocytes, (15 to 20/u.), containing fat and 1 to 4 or more proteid globules. 



Besides these regularly formed constituents of the milk, its 

 sediment contains flaky constituents, small irregular shaped coag- 

 ula, which readily tinge with basic anilin dyes, or with nuclear 

 staining substances. Frequently they are without any structure. 

 At times they appear in individual milkings, almost completely 

 dominating the microscopical field. They are the early stages of 

 the corpora amylacea, soon to be described, which appear either 



* 







' 



Budding globules, free nuclei, Nissen's globules, that is 



cell fragments, in the sediment of cow's milk. 



1 X 1000. 



