THE CLASSIFICATION OF BACTERIA 53 



ft Stained by Gram's method. B. muripestifer Group. 

 ** Gelatin liquefied. 



t Gas generated in glucose bouillon. B. Cloaca Group. 

 ft No gas generated in glucose bouillon. Include a large num- 

 ber of bacteria not sufficiently described to arrange in 

 groups. 

 b. Gelatin colonies ameboid, cochleate, or otherwise irregular. 

 * Gelatin liquefied. Proteus vulgaris Group. 

 ** Gelatin not liquefied. B. Zopfi Group. 

 II. Produce endospores. 



A. Aerobic and facultative anaerobic. 



1. Rods not swollen at sporulation. 



a. Gelatin liquefied. 



* Liquefaction of the gelatin takes place slowly. Ferment urea, 

 with strong production of ammonia. 



Uro-bacillus Group of Miquel. 

 ** Gelatin liquefied rather quickly, 

 f Potato cultures rugose. Potato Bacillus Group. 

 ft Potato cultures not distinctly rugose. B. Subtilis Group. 



b. Gelatin not liquefied. B. soli Group. 



2. Rods spindle-shaped at sporulation. B. licheniformis Group. 



3. Rods clavate at sporulation. B. sublanatus Group. 



B. Obligate anaerobic. 



1. Rods not swollen at sporulation. Malignant OZdema Group. 



2. Rods spindle-shaped at sporulation. Clostridium Group. 



3. Rods clavate-capitate at sporulation. Tetanus Group. 



Pseudomonas Migula 



I. Cells colorless, without a red-colored plasma and without sulphur granules. 

 A. Grow in ordinary culture media. 

 I. Without endospores. 



a. Aerobic and facultative anaerobic. 

 * Without pigment, 

 f Gelatin not liquefied. 



Gas generated in glucose bouillon. 



Ps. monadiformis Group. 

 No gas generated in glucose bouillon. 



Ps. ambigua Group. 



