134 BACTERIOLOGY 



Bouillon. Turbid, with a whitish sediment. 



Milk. Not coagulated. In gelatin and agar much gas. 



Pathogenesis. Mice die in 1-3 days of septicaemia; guinea pigs and rabbits 



refractory. 

 Habitat. Isolated from the blood of a mouse which had been inoculated with 



the sputum of a pneumonia patient. 



29. Bact. limbatum Marpmann 



Bact. limbatum-acidi-ladici Marpmann : Erganzungshefte des Centralblatt f. allgemeine 

 Gesundheitspflege, II, 122. 



Morphology. Bacilli short, thick, with a capsule. 



Milk serum gelatin colonies. 24 hours, punctiform, white, glistening ; edges 



sharp. 

 Gelatin stab. Slight growth in depth ; surface growth white, flat. 

 Litmus milk. 24 hours coagulated, slightly reddened. Grow at 37 C. 

 Habitat. Milk. 



30. Bact. nasalis 



Vorltommen von Frisch'schen Bacillen in der Nasenschleimhaut des Menschen u. der 

 Thiere: Simoni, Centralblatt f. Bakteriol., XXV, 1899,625. 



Morphology. Bacilli rather large, oval, with a thick capsule, which commonly 

 encloses two rods. In cultures smaller, more rod-like, and without a 

 capsule. Stain readily. 



Gelatin colonies. Round, much raised, homogeneous, opalescent, waxy ; 

 growth viscous. 



Gelatin stab. Good growth in depth ; no gas. Surface growth raised convex, 

 opalescent, becoming dirty white, never porcelain-white. 



Agar slant. In 24 hours a moist, glistening, translucent, watery streak. 



Glycerin agar colonies. 24 hours, 37 . Deep : dark, small, opaque. Surface: 

 largest, the size of a pin's head, raised, translucent, whitish-grayish. 



Bouillon. In 24 hoars at 37 , a dense turbidity, with a delicate pellicle on the 

 surface. 



Milk. Not coagulated, not acid. 



Potato. A raised, translucent, colorless, watery, glistening streak. No develop- 

 ment in acid media. No gas in glucose bouillon. 



Pathogenesis. Not pathogenic to guinea pigs and rabbits, except an infiltra- 

 tion at the point of injection. 



Habitat. Isolated from nasal secretions in rhinoscleroma. 



