150 BACTERIOLOGY 



74. Bact. acidiformans Sternberg 



Manual 0/ Bacteriology, 1892, 449. 



Morphology. Bacilli short, thick rods to longer forms and filaments. 

 Gelatin colonies. Deep: round, opaque, homogeneous. Surface: round, 



translucent opaque, slightly irregular and somewhat iridescent. 

 Gelatin stab. Like Bact. pneumonia ; gas in the gelatin. 

 Agar slant. Growth glistening, creamy. In glycerin agar, much gas; 



medium becomes intensely acid. 

 Potato. Growth thick, milky white, semi-fluid. 

 Glycerin bouillon. A milky opacity, and much gas. A slight reduction of 



nitrates to nitrites. 

 Litmus milk. Reddened. 

 Pathogenesis. Intraperitoneal inoculations of guinea pigs and rabbits, 1 .0-2.0 cc, 



cause death in 24 hours ; bacilli in the blood in small numbers ; spleen 



enlarged ; intestines hyperaemic. 

 Habitat. Isolated from the liver of yellow fever cadaver. 



75. Bact. astheniae Dawson 



U. S. Dept. Ag., Bureau of Animal Industry, 1898, 330. 



Morphology. Bacilli 0.5 : 1 .0-1 .3 fi ; ends rounded ; occur singly and in pairs- 

 Do not stain in acid or alkaline methyl-blue or in carbol-fuchsin, but do> 

 stain well in aqueous solutions of the latter. Stain by Gram's method. 



Gelatin colonies. Deep : yellowish, with irregular lobulate margins. Surface .- 

 in 24 hours, 1.5 mm., round, raised, papillate in centre, with yellowish 

 borders. 



Gelatin stab. In depth, yellowish, discrete, closely packed colonies ; on the- 

 surface, a brownish, spreading, deeply dentate growth, with delicate 

 periphery. 



Bouillon (acid). A dense turbidity, with pellicle on the surface. Medium- 

 yellowish green, with a putrefactive odor. Alkaline bouillon rendered 

 slightly acid. 



Agar slant. A good, white, opaque growth along line of stroke. 



Potato. In 3 days a yellowish, creamy growth, with gas blisters. 



H \ 

 Glucose bouillon. Rendered acid ; much gas. = =. 



CO, 2 



H 2 



Lactose bouillon. Rendered acid ; moderate gas production. -^- = -. 



H 2 

 Saccharose bouillon. Rendered acid ; but little gas. ~=p = -. 



