160 BACTERIOLOGY 



Agar colonies. Star-shaped, with broad, radiating outgrowths. 

 Bouillon. Not turbid, with a membrane. 

 Agar slant. Growth grayish. 

 Potato. Raised dry colonies. 

 Habitat. Water. 



103. Bact. aquatilis (Zimmerman) 



B. radiatus-aquatilis Zimmerman : Bak. Nutz u. Trinkwasser, 1890. 



Morphology. 0.6 : 1 .2-2.5 /* 



Gelatin colonies. Surrounded by a delicate " Strahlenkranz." 



Gelatin stab. A saccate liquefaction, with a pellicle on the surface ; medium 



turbid. 

 Agar slant. Growth gray, translucent. 

 Potato. Growth yellowish. 



104. Bact. flexuosum (Wright) 



B. flexuosus Wright : I.e., 460. 



Morphology. Bacilli medium-sized, thick, rounded ; also chains and filaments. 

 Gelatin colonies. Deep: round oval, brownish, granular. Surface: in 2 



days, whitish irregular clumps. Microscopically, twisted strands, dense 



in centre, edge irregular. 

 Gelatin stab. A crateriform liquefaction, becoming stratiform ; medium 



alkaline. 

 Agar slant. Growth grayish, translucent, limited ; the agar becoming slightly 



greenish. 

 Bouillon. Turbid, becoming slightly greenish. 

 Potato. Growth brownish, viscid, glistening, uneven. 

 Litmus milk. Coagulated, acid. Indol, a slight or doubtful reaction. Grow 



at 36 . 

 Glucose bouillon. Gas not produced. 

 Habitat. Water. 



105. Bact. ambiguum Chester 



Del. College Ag. Expt. Sta. Report, 1899. 



Morphology. On agar and potato, short rods and ovals to longer forms 3-4 

 times their breadth ; 0.7 : 1.0-3.0 p. In neutral bouillon long filamentous 

 forms appear with often involution forms. Stain with ordinary analine 

 colors ; decolorized by Gram's method. Flagella absent. Aerobic, grow 

 only in open end of fermentation tube. Grow at 37 C. 



