If)0 BACTERIOLOGY 



Gelatin stab. Liquefaction shallow, craferiform ; lined with bacterial growth ; 

 from the base of the latter filaments radiate a short distance into the 

 gelatin ; a barely perceptible growth in depth ; liquefaction becoming 

 stratiform. 



Agar slant. Growth dense, white, smooth. 



Potato. Growth thick, grayish white, dull, and mealy. 



Habitat. Sea-mud, Woods Hole, Mass. 



187. Bact. sputi 



Bacillus No. 4 Pansini : Virchow's Archiv, CXXII, 1890. 



Morphology. Bacilli large, in long filaments. 

 Gelatin colonies. Radiate. 

 Potato. Growth of moist, dew-like drops. 

 Bouillon. Turbid, with a dense membrane. 

 Habitat. Sputum. 



188. Bact. sessile (Klein) 



B. sessilis Klein : Centralblatt f. Bakteriologie, VI, 1889, 10. 



Morphology. Bacilli like anthrax ; spores, in size and form, like B. sublilis, 



but show polar germination. 

 Bouillon. With a membrane. 

 Pathogenesis. Non-pathogenic to mice. 

 Habitat. Isolated from the blood of a cow supposed to have died of anthrax. 



189. Bact. anthracis (Cohn) Migula 



First discovered by Rayer : Memoirs de la Soc. de Biol., 1850, 141. 



Les infusoires de la maladie charbonneuse Davaine : Compt. rend., LXIX, 1864, 393. 



Bacteridie du charbon Pasteur-Joubert : Compt. rend., LXXXIV, 900. 



B. anthracis Cohn: Beitrage Biol., I, 2 Heft, 1875, 177. 



Bact. anthracis Migula: Engler-Prantl, Die Natiirlichen Pflanzenfamilien, 1895. 



Morphology. Bacilli 1 .1-1.2 : 3-10 p ; ends square, in chains ; in animal body, 



with a capsule. Stained by Gram's method. 

 Gelatin colonies. Round, white, becoming in 3-4 days liquefied ; contents 



turbid; microscopically show grayish centres, with clearer floccose 



borders. 

 Gelatin stab. An arborescent growth, becoming a crateriform to saccate 



liquefaction ; no membrane on the surface. 

 Agar colonies. Very characteristic, floccose. 

 Agar slant. Growth gray white, moist, glistening, mealy ; older cultures 



show pellucid dots. 



