192 BACTERIOLOGY 



Agar slant. Growth thin, white, spreading, membranous. 

 Potato. At 37 , dirty white, milky colonies, becoming confluent. 

 Pathogenesis. Subcutaneous inoculations of rabbits and guinea pigs show a 



gangrenous process, with death in 2-3 days. 

 Habitat. Isolated from a case of senile gangrene. 



194. Bact. Markusfeldii 



B. der trichorrkexis-nodosa Markusfeld : Centralblatt f. Bakteriol., XX, 1897, 230. 



Morphology. Bacilli 0.4-0.6: 1.7-2.2 (jl. Grow at 39. 



Gelatin colonies. Roundish clumps, which quickly liquefy the gelatin. 



Gelatin stab. In 48 hours, a semi-spherical liquefaction of the gelatin. 



Agar colonies. In 24 hours, at 37 . Deep colonies : round, oval, white, brown- 

 ish yellow ; contour irregular, with ringlet-like outgrowths and small 

 colonies from border. Surface colonies : white, opaque in centre ; border 

 granular to floccose. 



Agar slant. White, moist colonies. 



Milk. Coagulated. 



Potato. Growth moist, white. 



Habitat. Associated with this disease. 



195. Bact. crinatum (Wright) 



D. crinatus Wright : I.e., 453. 



Morphology . Bacilli large, chains to segmented threads. 



Gelatin colonies. Deep : dark opaque, round to oval, entire, granular margins. 



Surface: in 2 days, 1-2 mm., round, glistening, translucent, entire; in 



3 days, dense, felt-like margins, crimpled, slightly sunken ; margins also 



fimbriate to frayed. 

 Gelatin stab. Liquefaction broadly funnel-formed to stratiform. 

 Agar slant. Growth gray white, frosted. 

 Bouillon. Turbid, with white floculi. 

 Potato. Growth thick, creamy-white, viscid, spreading, becoming yellowish, 



caseous. 

 Litmus milk. Decolorized, amphoteric, becoming viscid, yellowish, caseous. 

 Glucose bouillon. No gas. Indol slight or doubtful. Grow at 37 . 

 Habitat. Water. 



196. Bact. verticillatum Ravenel 



B. verticillatus Ravenel : I.e., 13. 

 Morphology. Bacilli thick, 3-5 times their breadth, rounded. Spores only 

 in potato cultures after 2 weeks. Facultative anaerobic. 



