THE CLASSIFICATION OF BACTERIA 20 



10. B. Poelsii 



VUeschvergiftung te Rotterdam Poels-Dhont: Tweede rapport van de des Kundigen. 



Morphology. Bacilli slowly motile. Grow on gelatin like B. coli. A very- 

 weak gas development in glucose bouillon. No gas in lactose and sac- 

 charose bouillon. 



Bouillon. Rendered alkaline. Indol produced. 



Milk. Not coagulated. 



Pathogenesis. Intravenous inoculations of cows with 3 gelatin cultures re- 

 sulted in death in 14 hours ; bacilli in all organs and in the muscles. 



Habitat. Isolated from beef in meat poisoning. 



11. B. columbarum 



Bacillus of pigeon cholera Moore : U. S. Dept. of Ag., Bureau of Animal Industry, 



Bull. No. 8, 1895. 



Morphology. Bacilli 1.0: 1. 0-1.6 /1; size variable in different media; ends 

 rounded ; in the tissues, usually in pairs. Flagella not more than 8. 



Gelatin colonies. Surface : small grayish dots ; microscopically, yellowish, 

 granular. 



Agar slant. At 37 , growth grayish, glistening, not viscid. 



Agar colonies. Convex, entire, 0.5-1.5 mm. 



Potato. Growth thin, glistening, slightly yellowish. On acid potato no growth. 



Alkaline bouillon. In 24 hours, turbid-, slightly acid, with a thin, grayish 

 membrane, becoming alkaline. In acid bouillon, only slight growth, reac- 

 tion unchanged. 



Milk. Not coagulated, strongly alkaline. 



Glucose bouillon. Gas ; H : C0 2 : : 2 : 1. No gas in lactose or saccharose 

 bouillon ; media rendered alkaline. Indol produced. 



Pathogenesis. Intravenous inoculation of rabbits with 0.3 cc. of bouillon 

 culture causes congestion of the internal organs. Intestinal mucosa red- 

 dened in patches. Subcutaneous inoculation causes death in 4-5 days, 

 with purulent infiltration at the point of inoculation ; in the liver, necrotic 

 spots ; spleen enlarged, dark-colored, and friable. Subcutaneous inocula- 

 tion of guinea pigs, 0.1-0.2 cc, causes death in 8-10 days. Pathogenic 

 to pigeons. Differs from hog cholera (1) bacilli larger; (2) in bouillon 

 a delicate membrane, and in old cultures a deposit on the sides of the 

 tube ; (3) a marked indol reaction ; (4) it is less rapidly fatal in small 

 doses for experimental animals, and the lesions produced in rabbits are 

 comparable to those following the inoculation of the more attenuated 

 varieties. (Moore, I.e.) 

 p 



