226 BACTERIOLOGY 



Gelatin stab. In depth, growth pearly; on the surface growth milky-white. 

 Nitrate bouillon. In 2 days at 30 a strong turbidity, and a membrane on the 



surface ; nitrates reduced. 

 Habitat. Isolated from cow and guinea pig manure. 



61. B. agilis Amp.-Gar. 



B. denitrificans-agilis Ampola-Garino : Centralblatt f. Bakteriol., II, 2te Abt., 1896, 673; 

 Jensen, I.e., IV, 1898, 408. 



Morphology. Bacilli 0.3-0.5 : 1.0-2.5 ih or smaller. No polar stain. 

 Gelatin colonies. In 5 days scarcely visible ; white, entire, homogeneous. 

 Agar colonies. Small, white, slimy, not spreading. 

 Agar slant. Growth grayish white, slimy, limited, or spreading where the 



medium is moist. 

 Gelatin slant. Growth in 6-8 days grayish white, slimy, limited, somewhat 



knobby. 

 Gelatin stab. In depth, growth pearly ; on the surface the growth in 8 days 



is grayish white, 1 mm. in diameter. 

 Nitrate bouillon. In 1-2 days turbid, with a membrane; nitrates reduced. 

 Habitat. Isolated from cow manure. 



62. B. Hartlebii (Jensen) 



Bad. Hartlebii Jensen : Centralblatt f. Bakteriol., IV, 2te Abt., 1898, 449. 



Morphology. Bacilli on agar and in nitrate bouillon 0.7:2-3-4/11. Polar 



stain rare. 

 Gelatin colonies. In 4-5 days the deep colonies are small, white, entire ; the 



surface colonies, 1 mm., white, translucent, with entire or erose borders. 



In 2-3 weeks the colonies are watery, slimy, and 1-3 mm. in diameter. 

 Gelatin stab. In depth, a pearly growth ; on the surface the growth is white, 



slimy, and 3-6 mm. in diameter. 

 Gelatin slant. Growth white, moist, glistening, raised, limited. 

 Agar slant. In 2-3 days at 30 growth thick, grayish white, moist, glistening, 



watery. 

 Agar colonies. In 3 days at 30 the deep colonies are small, white, entire ; the 



surface colonies translucent, watery. 

 Nitrate bouillon. Rendered strongly turbid, with a membrane ; nitrates 



reduced. 

 Habitat. Soil. 



