THE CLASSIFICATION OF BACTERIA 289 



Litmus milk. Color discharged in 12 days, alkaline, not coagulated. 

 Glucose bouillon. No gas. Indol negative. Grow at 36 . 

 Habitat. Soil. 



229. B. saprogenes Kramer 



B. saprogenes-vini No. VI Kramer : Bakteriologie Landwirtsch., 1890, 139. 



Morphology. Bacilli 1.0: 2.0 p. Obligate aerobic. 



Gelatin stab. Surface growth dirty white ; liquefied rapidly. Ammonia 



developed in old cultures. 

 Habitat. Isolated from diseased wine. 



230. B. Baccarinii Macchiati 



Centralblatt f. Bakteriol., IV, 1898, 332. 



Morphology. In gelatin cultures rods 0.7-0.8:2.0-3.5 p. straight slightly 

 curved; occur singly, in chains, and as zoogloea. In bouillon, long 

 filaments like Leptothrix. Flagella peritrichic. Rods swollen in the 

 middle at sporulation, oval. Spore germination polar. 



Gelatin stab. In 4-5 days, an empty shallow funnel, beginning with an air 

 bubble above, lined with a white growth, with a membrane on the lique- 

 fied gelatin. The gelatin of old cultures is colored black, and is fluores- 

 cent. 



Agar slant. Raised, yellowish colonies, becoming gray, spreading. 



Milk. In 3-4 days, coagulated ; in 14 days, peptonized, acid. 



Potato. Growth light yellow, becoming straw yellow ; potato liquefied or 

 softened. Optimum temperature 23-25 , maximum 40 . 



Habitat. Associated with mal-nero of the vine. 



231. B. Duclauxi Miquel 



Urobacillus Duclauxi Miquel : Ann. Micrographie, II, 1889, 58. 

 Morphology. Bacilli 0.6-0.8 : 2-10 fi; chains. Gelatin slowly softened. 

 Bouillon (made alkaline with NH 4 HO). Turbid, becoming viscous ; bad odor. 

 Habitat. Isolated from canal and river water. 



232. B. cinctus Ravenel : I.e. 



Morphology. Bacilli straight, variable, involution forms. Rods show deeply 

 stained spots. 



Gelatin colonies. In 24 hours, minute, yellowish, granular, entire ; in 72 hours, 

 0.5 mm., nucleus surrounded by a yellowish zone, then a gray-veined 

 zone, with irregular margins. In 8 days, 1 mm., grayish white, round, 

 elevated, entire, finely veined mottled, often with ferny outgrowths, 

 u 



