THE CLASSIFICATION OF BACTERIA 299 



255. B. cuneatus 



B. Anaerobic No. Ill Flugge : Zeitsch. f. Hygiene, XVII, 1894, 272. 



Morphology. Bacilli long; cuneate at sporulation. 



Gelatin colonies. Yellow brown ; contour sharp ; border irregular. 



Agar colonies. Dark brown, irregular, lacerate. 



Glucose bouillon. Turbid; gas; rancid odor. 



Milk. Unaltered in 8 days. 



Habitat. Milk. 



256. B. sporogenes Klein 



B. enteritidis-sporogenes Klein: Centralblatt , Bakteriol., XVIII, 1895, 737, XXII, 578, 

 XXIII, 542,913. Report Loc. Gov. Board, Supplement, 1897-1898, 210. 



Morphology. Bacilli 0.8:1 .6-4.8 fi filaments chains. Stained by Gram's 

 method. Form oval spores, mostly polar, with clavate enlargement of 

 the rod. Flagella mostly at one side of an end, in bundles. 



Glucose gelatin colonies. Uniform, spherical, finely granular, translucent 

 masses of liquefied gelatin. 



Agar colonies. Round, gray disks, quite opaque in centre and granular at 

 border. 



Blood serum. Growth thin, gray ; medium liquefied ; fluid turbid, alkaline, 

 and stinking. 



Glucose bouillon. Turbid ; often no growth. 



Potato. Anaerobically, at first no growth ; in 8-14 days, a number of small 

 round yellowish colonies. 



Formate of soda agar colonies. Small, flat, gray, with dark granular centres 

 and clear sharp borders. Grow best in milk, becomes translucent, pep- 

 tonized, with much gas. 



Pathogenesis. Virulent cultures become greatly attenuated in 3-4 generations. 

 Subcutaneous inoculations of 1 cc. of a milk culture into guinea pigs 

 cause death in 18-24 hours. Strong oedema, muscular tissue strongly 

 infiltrated, and a bloody stinking cedematous fluid. Bacilli sparingly 

 present in heart, blood, and spleen. 



Habitat. Widely distributed sewage, water, horse and cow manure, street 

 dust. 



257. B. spinosus Ltideritz 



Zeitsch. f. Hygiene, V , 1888, 152. 



Morphology. Bacilli 0.6 : 4-8 /*. Spores at end of rod where the latter is 

 swollen. No starch reaction with iodine. 



