THE CLASSIFICATION OF BACTERIA 333 



** Liquefaction of the gelatin takes place in depth, cholera-like. 



12. Microspira tyrogena (Dencke) Migula. 

 3. Indol reaction not stated. 



Microspira marina Russell, see No. 20. 

 B. Gelatin not liquefied, or only slightly so in No. 20. 



1. A slight liquefaction of the gelatin at the surface in stab cultures. 



13. Microspira choleroides (Bujwid). 



2. Absolutely no liquefaction of the gelatin. 



a. Growth in gelatin plates slow, colonies minute. 



14. Microspira Weibeli. 



15. Microspira denitrificans (Sewerin). 



b. Colonies on gelatin plates of average size. 

 * Potato cultures becoming brownish. 



16. Microspira saprophile (Weibel). 



17. Microspira cloaca. 



** Potato cultures yellowish white. 



18. Microspira terrigena (Giinther). 



1. Microspira phosphorescens (Fischer) 



B.phosphorescens Fischer: Zeitsch. f. Hygiene, II, 1887. 



fhotobact. indicum Beijerinck : Akademie van Wetenschappen Afdeeling Natuurkunde z. 



Reeks, VII, Amsterdam, 1890. 

 B. phosphor escens-indicus Kruse : Fliigge, Die Mikroorganismen, 1896, 330. 



Morphology : Bacilli 0.6-0.8 : 2.0 fj. bent filaments. 



Gelatin colonies. Deep round, entire, bluish sea green. Surface : 



granular, brownish, borders undulate, sinking slowly in the liquid 



gelatin. 

 Gelatin stab. Slight growth in depth ; on the surface, liquefaction napiform, 



with an air bubble. 

 Agar slant. Growth grayish white. 

 Potato. No growth except when cooked in salt water. Phosphorescence 



bluish, disappearing in old cultures. Grow well in sea water on fish, 



meat, blood, and egg, with a bluish phosphorescence. Non-pathogenic. 

 Habitat. Isolated from phosphorescent sea water, West Indies. 



2. Microspira Fischeri (Beijerinck) 



Photobact. Fischeri Beijerinck : I.e. 



B. phosphor escens-indigenus Kruse : Fliigge, Die Mikroorganismen, 1896,331. 



Cultural characters as before. Differs in liquefying gelatin rather more slowly, 

 phosphorescence less intense, the latter absent when grown on fresh 

 media, as above. 



Habitat. Sea water. 



