THE CLASSIFICATION OF BACTERIA 337 



Bouillon. Turbid, with a thin pellicle. 



Pathogenesis. Non-pathogenic to guinea pigs. 



Habitat. Well water. 



() Vibrio choleroides, /8. Bujwid : Centralblatt f. Bakteriol., XIII, 1893, 120. 



Morphological and cultural characters like Msp. comma, but more anaerobic 



in habit, and forms a deeper liquefied funnel. 

 Habitat. Water. 

 (C) Vibrio Ivanoff Kruse : FlUgge, Die Mikroorganismen, 1896, 592. Noted 



by Ivanoff: Zeitsch. f. Hygiene, XV. 

 Bacilli fine and elongated, slightly curved comma forms ; otherwise identical 



with Msp. comma. Ivanoff considers it only a morphological variety. 

 Habitat. Isolated from the dejecta of typhoid patients. 



7. Microspira danubica (Heider) 



Vibrio danubicus Heider: Centralblatt f. Bakteriol., XIV, 1893, 34 1 - 



Morphology . Bacilli like Msp. comma. Gelatin rapidly liquefied, crateriform 



funnel-formed. 

 Gelatin colonies. When the gelatin layer is thick, colonies like Msp. comma', 



when thin, surface colonies flat, spreading, irregular, with undulate 



coarsely lobate borders. 

 Milk. Coagulated. 

 Potato. At 37 growth brownish, scanty. Indol positive. Pathogenic for 



guinea pigs, slightly so for pigeons. A negative reaction with Pfeiffer's 



serum. 

 Habitat. Isolated from canal water. 



8. Microspira Berolinensis (Neisser). Migula 



Vibrio Berolinensis Neisser: Archiv f. Hygiene, XIX, 1893. 



Morphology. Bacilli like Msp. comma, but somewhat smaller. A single 

 polar flagellum. Not stained by Gram's method. 



Gelatin colonies. Like Msp. comma, but not much granulated or fragmented. 

 Liquefaction of the gelatin very slight. Deep colonies: small, round, en- 

 tire, colorless, transparent, slightly granular. Surface colonies: small, 

 thin, membranous, with a round central nucleus ; no depression is formed, 

 and the edges remain sharp. 



Gelatin stab. Growth like Msp. comma, but a slower growth. 



Agar slant and potato. Growth like Msp. comma. 

 z 



