338 BACTERIOLOGY 



Bouillon. Growth more rapid han Msp. comma; medium rendered alkaline. 

 Milk. Not coagulated, not rendered acid. 

 Habitat. Isolated from filtered river Spree water. 



9. Microspira maasei (v. Hoff) 



Spirillum maasei v. Hoff: Cenlralblatt f. Bakteriol., XXI, 1897, 797- 

 Morphology. Bacilli short, thick, 1. 0-1.2 \l. One or two polar flagella. Spi- 

 rals of 1-2 turns. 

 Agar slant. Growth milky white. 

 Milk. Not coagulated. 



Bouillon. A membrane on the surface ; no acid production. Indol produced. 

 Habitat. Isolated from Rotterdam water. 



10. Microspira protea (Buchner) 



Vibrio der cholera-nostras Finkler-Prior : Centralblatt f. allg. Ges. Erganzungshefte, 



Bd. I, 1884. 

 Vibrio proteus Buchner: Sitzungsber. d. Ges. f. Morph. u. Physiol., Miinchen, I, 21. 



Morphology. Bacilli 0.4-0.6: 2.4 fx, more or less curved, rather longer and 

 thicker than Msp. comma, and often pointed at the ends and thicker in 

 the centre. S forms and spirals less common than in the case of Msp. 

 comma. 



Gelatin colonies. In early stages like cholera, but grow rather more vigorously, 

 and the gelatin liquefied more rapidly. Microscopically, round, entire, 

 yellow, finely granular, often concentrically zoned. Colonies retain their 

 regular form and finer structure better than with cholera. 



Gelatin stab. Gelatin liquefied much more rapidly than with cholera ; a sac- 

 cate liquefaction in 24 hours, with turbid contents. 



Milk. Coagulated, becoming liquefied (Lehmann-Neumann) ; unchanged 

 according to other authors ; slightly acid. 



Potato. Growth at room temperature, within 48 hours, slimy, grayish yellow. 

 {Msp. comma does not grow on potato at room temperatures.) 



Glucose bouillon. No gas. H 2 S production slight. Cultures have a foetid 

 odor. 



Agar slant. Growth dirty yellowish (cholera whitish). 



Plovers egg albumen. A bright yellow growth (cholera whitish). See Pear- 

 main-Moor, "Applied Bacteriology," 1892, 205. No indol reaction after 

 3 days. 



Pathogenesis. On experimental animals action like Msp. comma, but some- 

 what milder. 



Habitat. Isolated from stools in cholera-nostras, cholera infantum, etc. 



