THE CLASSIFICATION OF BACTERIA 339 



Varieties. 



(A) Vibrio Lissabonensis Pestana-Bettencourt : Centralblatt f. Bakteriol., XVI, 



1894, 401. 

 According to Chantemesse identical, or nearly so, with the preceding. From 



descriptions, indistinguishable from the latter. 

 Habitat. Isolated from a case of choleraic disease in Lisbon. 

 () Vibrio helicogenes Fischer: Centralblatt f. Bakteriol., XIV, 1894, 73. 



From descriptions, indistinguishable from Msp. protea. 

 Habitat. Isolated from the stools of a sick woman. 



11. Microspira Gindha (Kruse) 



Vibrio Gindha Kruse : Fliigge, Die Mikroorganismen, 1896, 590. 



Morphology. Bacilli rather long curved rods, rather smaller than Msp. 

 comma. A single polar flagellum. 



Gelatin colonies. Cholera-like. In pepton solution in 24 hours, at 37 , a 

 negative or only a faint indol reaction. 



Pathogenesis. Subcutaneous inoculations into pigeons and guinea pigs nega- 

 tive. Living or dead cultures show toxic properties when inoculated 

 intraperitoneally into guinea pigs. 



Habitat. Isolated by Pasquale from water. 



12. Microspira aquatilis (Giinther) 



Vibrio aquatilis Giinther: Deutsche med. Wochenschrift, 1892, 1124. 



Morphology. Bacilli like Msp. comma. A single polar flagellum. 



Gelatin colonies. Round, entire, brownish, finely granular. 



Gelatin stab. Growth only on the surface, becoming a crateriform liquefaction. 



Agar slant. Growth like cholera. 



Potato. No growth either at 20 or 37 . 



Bouillon. No growth in alkaline or amphoteric bouillon at 37 ; at 20 , only a 



faint growth after some weeks. Indol negative. A strong odor of H 2 S. 



Non-pathogenic. 

 Habitat. Isolated from water of river Spree. 



13. Microspira tyrogena (Dencke) Migula 



Spirillum tyrogenum Dencke : Deutsche med. Wochenschrift, 1885, No. 3. 

 Msp. tyrogertu Migula: I.e. 



Morphology. Bacilli like Msp. comma, but rather smaller and more slender, 

 often very long spirals with close windings. 



