358 BACTERIOLOGY 



Pathogenesis. Intraperitoneal inoculations into guinea pigs, of butter contain- 

 ing the preceding bacilli, result, in 3-4 weeks, in peritonitis, with abundant 

 tubercles on the peritoneum, which have cheesy, purulent centres. Similar 

 tubercles may be found on'the pleura and in the spleen. These tubercles 

 lack the microscopic characters of true tubercles in the absence of giant 

 cells, but more closely simulate glanders nodules. They contain the 

 bacilli in large numbers, from which pure cultures can be readily obtained. 



Habitat. Found by Rabinowitsch, Petri, and others in butter and milk. 



17. Mycobact. Moelleri 



Timothee Bacillus or Grass Bacillus I Moeller: Wiener med. Wochenschr., 1898, p. 2358. 



Morphology. Bacilli 0.2-0.5 ? x -4 M.j often bent ; often in chains of 2-3, or in 

 clumps ; also as filaments with clubbed ends, or branched. Show irreg- 

 ular staining like tubercle bacilli, and retain the red color of carbol 

 fuchsin when treated with acid and acid alcohol. 



Glycerin agar. At 37 , after several days, grayish white, dry, scaly colonies. 



Bouillon. Small granular masses on the walls and bottom of the tube; on 

 the surface, a yellowish membrane. 



Milk. On the surface, yellowish spots or colonies, or a yellowish ring adherent 

 to the walls. 



Potato. A warty layer like tubercle bacilli. 



Pathogenesis. Pathogenic to rabbits and guinea pigs, producing a pseudo- 

 tuberculosis as in Mycobact. butyri. 



Habitat. Isolated by Moeller from infusions of timothy grass. 



The Mist Bacillus of Moeller, isolated from cow manure, is a closely related 

 organism (Berliner thierarztl. Wochenschr., 1898, 100). In morphology it 

 simulates the tubercle bacillus ; also occurs as long filaments ; also clubbed 

 at one or both ends, and without branching. Does not grow in milk. 

 Grows well on glycerin agar. 



18. Mycobact. graminis 



Grass Bacillus II Moeller : Centralblatt f. Bakteriol., XXV, 1899, 369. 



Morphology. Bacilli in fluid media mostly as rods whose morphology an ' 

 staining properties simulate tubercle bacilli. In old cultures, often fila- 

 ments and branched forms. On solid media, at first only rods and coccoid 

 forms; later, filaments showing true branching. Rods 0.2-0.4: 1-5 /t. 

 Stain by Gram's method. 



