44 



THE PROPERTIES OF PROTEINS 



of hydrion of 1-5 x io~ 6 was found when the pressure was at a 

 minimum. 



Table 13. 

 Osmotic Pressure of i per cent. Albumin at 18. 



A more complete series for glutin gave the following results : 



Table 14. 

 Osmotic Pressure of i per cent. Glutin at 25. 



At the point of maximum precipitation of albumin by alcohol 

 (or of maximum fluidity) a hydrion concentration of 1-95 X io- 6 

 with a concentration of chlorine > io~ 4 was found to obtain.* 



* In a later research of Pauli and T. Oryng (Biochem. Zeitsch., 1915, 

 76, 373) it is shown that using i per cent, serum albumin in a calomel 

 electrode for determining the concentration of chlorions, a chlorine 

 concentration of about 3 x io- 4 normal is developed compared with 

 the solubility of mercurous chloride in water, i x io- 8 N. This 

 increase in [Cl 7 ] is due to formation of complex chlorides by the calomel 

 and the albumin. On addition of acid, the mercurous ion is displaced 

 from the albumin, and hydrion takes its place. In the above case we 

 should equate the chlorion concentration, which arises from the salt 

 formation between the HC1 and the protein, to the normality of the acid 

 added. For the hydrion is practically completely combined, while the 



