5.4 LTS OF ALBUMIN AND ACIDS 



57 



Bugarsky and Liebermann worked on egg albumin dialysed 

 free from salts, and on mixtures of albumoses purified in a 

 similar way. Their measurements of the hydrion concentra- 

 tion are, however, more troublesome and less reliable than 

 those more recently carried out by S. P. L. Sorensen and 

 Hasselbalch with much improved technique. The following 

 table gives an example of the results of Bugarsky and Lieber- 



mann : 



Table 17. 



= gm. albumin to 100 cc. 0-05 N Hydrochloric Acid.) 



These figures, which are displayed graphically in Fig. 9, 

 show that on addition of more than 6 gm. albumin to 100 c.c. 

 of 0-05 N HC1, the 

 acid combines 

 pletely with it. 



iOu 



corn- 

 Also, 



with 3 gm. albumin 

 and lesser quantities, 

 the H' ions and Cl 7 

 ions are taken up in 

 equal number, but 

 above that concentra- 

 tion of protein, the H- 

 ion is more .completely 

 combined than the Cr 

 ion. The authors 

 deduce from this the 



grar. 



c 



is ofji rotezn 



Z 3 * 5 

 FIG. 9 (Table 17). 



constitution of the compound of albumin and acid : " The 

 compound albumin HC1 dissociates in water to give Cl' ions and 

 albumin H- ions (the latter might be named ' albuminium ') ; 



