SALTS OF ALBUMIN AND ACIDS 



59 



undertaken with every precaution show that movement to 

 the anode alone occurs in acid albumin, indicating the presence 

 of only positive albumin ions. 



The ionisation of albumin-acid compounds has been made 

 quite clear by the careful measurements of H* and Cl' ion 

 concentrations by the potentiometer method undertaken by 

 K. Manabe and J. Matula.* Their results for an albumin 

 made from ox-blood serum are given below. The arrange- 

 ment is much clearer than that of Bugarsky and Liebermann, 

 for in this case the albumin concentration (about i per cent.) 

 was kept constant, the acid alone being varied. 



Table 18 (Fig. 10). 



Ox-serum Albumin (end concentration 1*09 per cent.) + HC1. 

 A. Hydrion Concentrations. 



B. C Morion Concentrations, 



It can be seen from these results that in lower concentrations 

 of acid practically all the H- ions are combined. With an 

 increase in the quantity of acid the combination tends to a 



* Biochem. Zeitsch., 1913, 52, 369. 



