CHAPTER IX 



SALTS OF THE GLOBULINS : MIGRATION VELOCITY 

 OF THE PROTEIN IONS 



CLOSELY connected with the work on the caseinates are the 

 researches of W. B. Hardy * on the salts of the globulins with 

 acids and with alkalis. This fundamental work is distinguished 

 by its modern point of view, and, on the chemical side, accurate 

 conceptions are brought to bear on the solution of the problem. 

 The globulin precipitate, obtained by slightly acidifying ox 

 blood serum diluted ten times with water, was carefully washed 

 for use in these experiments. 



Various acids and bases dissolve globulin to a differing 

 extent, and this property proved of great interest. A nephelo- 

 metric method of determination was used, a known opacity 

 being taken as the standard, for it was found that the solutions 

 suffered a gradual change in opacity. 



If the quantity in gram equivalents of hydrochloric acid 

 required to dissolve i gm. of dry globulin is taken as unity, the 

 values for various acids are those shown in the following table : 



Table 67. 



The strong monobasic acids, up to monochloracetic acid in 

 the table, show the same power of dissolving globulin. Weaker 



* " Colloidal solution : The Globulins," Jour. Physiol.,. 1905, 33, 

 251. 



