DISEASES OF THE OX AND SHEEP. 183 



been right in other points, the use of the material he recom- 

 mended is not advisable. 



Method of Inoculation. — The fluid should be obtained from 

 the interlobular tissue of a lung of an animal suffering from the 

 first stage of a mild attack, and as soon as possible after 

 slaughter. The parts which are dark red in colour and are 

 consolidated, and especially those parts which look gangrenous, 

 should be most carefully avoided. 



The fluid obtained should be of an amber colour, and should 

 be either fresh or at least preserved, so as to be intact and free 

 from the least decomposition. A piece of lung, distended with 

 this yellow semi-fluid substance, should be cut along the lymph- 

 channels, so as to allow this fluid to ooze out. It should then 

 be placed while still warm in a strainer over a suitable glass 

 bowl, covered over with flannel to keep it warm and clean. The 

 clear yellow liquid obtained should be preserved in glass tubes 

 about 4 in. long and f in. in diameter, which, when full, should 

 be hermetically sealed by a blow-pipe flame, unless the material 

 is required at once. The hair from the under surface of the 

 tail of the animal which it is desired to inoculate should be 

 clipped off" for about five inches from the tip forwards. Then 

 the skin should be cut transversely in two places, one distant 

 about an inch, and the other about three inches, from the tip of 

 the tail. A needle should then be pushed from the lower up- 

 wards to the higher incision, and the chaunel enlarged by a few 

 rapid movements. A piece of white thick worsted, previously 

 well-soaked in the yellow fluid, should be passed through the 

 eye of the needle, pulled through, and left in the skin as a small 

 seton, the ends being knotted so as to cause it to remain in 

 position. 



The way we have described is as nearly as possible, we believe,, 

 that of Mr. Rutherford, of Edinburgh, a skilled and successful 

 scientist and veterinary surgeon. Professor Williams recom- 

 mends that the skin should be slightly scarified, and that one 

 drop of the fluid should be injected. It is our belief that this 

 latter method, or rather the simple insertion of the fluid on the 

 exposed surface, is to be preferred. The Professor adds that 

 the upper surface should be inoculated, and that the tip of the 

 tail is selected because, in case of gangrene, which may some- 

 times occur, the tail may be easily amputated. About the fifth, 



