35G THE DISEASES AND DISOEDERS OF THE OX. 



1876, and when examined afresh after having been stained with 

 Loffler's methylen-blue they revealed the presence of numerous 

 micrococci, singly and as diplococci, and as short chains which 

 were found in the tissue of the skin papillse, and especially 

 between the deepest cells of the stratum malpighii, whence they 

 could be traced between the epithelial cells upwards towards the 

 superficial layers of this stratum. 



Dr. Klein has also proved by experiments that the action on 

 mice of the micrococcus scarlatinse derived from a human source 

 is the same as that from the cow, and also that house-mice are 

 more susceptible to its action than tame mice are ; and he also 

 states, basing his remarks on other experiments, that there can, 

 then, be no doubt that both by inoculation and feeding with 

 cultures of the micrococcus scarlatinse, derived from the 

 human source, a definite general disease in calves, cutaneous and 

 visceral, is produced, and that this disease bears a great resem- 

 blance to that observed in the Hendon cows, and observed also in 

 calves inoculated with the micrococcus derived from those cows. 

 However, it is to be observed that there were no ulcers on the 

 soft skin of the belly in these calves ; while, on the other hand, 

 the condition of the pericardium was not examined in the Hendon 

 cows. From these experiments we thus learn that with the 

 cultures of the micrococcus scarlatinee of a certain patient, both 

 by inoculation and by feeding, the identical and typical disease, 

 cutaneous and visceral, was produced in calves, and, further, 

 that the pericardial exudation contained the micrococcus 

 scarlatinse more abundantly than the blood, for while inocula- 

 tions made from the blood of the heart did not produce any 

 growth in three tubes, each of the three tubes inoculated with 

 the pericardial exudation yielded colonies of the micrococcus 

 scarlatinse. 



In all the animals which died or were killed in the course of 

 Dr, Klein's experiments, after they had been infected with the 

 micrococcus scarlatinse of one source or another, the condition 

 of the viscera was seen on microscopic examination to resemble 

 that found in the viscera of cases of fatal human scarlatina. Dr. 

 Klein has also found the scarlatina micrococcus in condensed 

 milk, and also that the cultures of this micrococcus had the same 

 effect as that derived from the Hendon cows and from the cases 

 of human scarlatina, and that it was successfully recovered by 



