HOW DOUBLE TREATMENT IS GIVEN 387 



either in the muscles of the hind limb or in the loose tissues back 

 of the ears or behind the foreleg. 



We are now ready to inject the virus. The dose of virus is 

 much smaller than that of serum, and runs from a few drops to 

 1^ c.c, according to the size of the animal. The correct amount 

 of the virus is now measured out, placed in the small syringe, 

 and injected in the opposite side from that where the serum was 

 injected. For instance, if the serum is injected into the muscle 

 of the right hind leg, the virus should be given in the muscle of the 

 left leg; while, if the serum be given behind the left ear, place the 

 virus under the skin behind the right ear. 



The skin at the point where the virus is to be injected should 

 be just as carefully cleaned and painted with tincture of iodin as 

 in the case of the location where the serum is to be injected. At 

 every step in the process cleanliness must be the watchword. 

 This will prevent many abscesses, many cases of blood-poisoning, 

 and other bad effects which are often blamed on the serum, when, 

 as a matter of actual fact, carelessness and lack of attention to 

 little points of importance is the cause of poor results. 



Do Not Mix Serum and Virus. — I have seen cases where men 

 injecting the serum and virus treatment simply mixed the virus 

 and serum together, and injected them both in the one place and 

 with the one syringe. This is not a proper method and should not 

 be followed. Give serum with the large syringe, and use this 

 syringe for no other purpose. Give the virus with the small 

 syringe, and use this instrument for this purpose only. Give the 

 injections at different points, and do not inject both serum and 

 virus in the one location. If a large dose of serum is needed on 

 account of the size of the animal, and it is necessary to use the 

 space back of both ears for the purpose of injecting same, then 

 inject the virus in some other part of the body, such as back of the 

 foreleg or in the muscle of the hind leg. 



Dose of Virus. — The dose of virus blood is, of course, very small. 

 It is to be remembered that this virus blood is, in fact, a pure cul- 

 ture of the hog-cholera virus or hog-cholera germ, and only a 

 few drops of it would be necessary to produce the disease in such 

 a severe form as to cause death. However, when injected with the 

 serum, a little more can be used without danger, as the germ- 



