78 



7. The lung was cut up in small piccef5 into a large basin, and left for about two hours to 

 drain ; the virus was then strained through two or three fine cloths, put into clear glass 

 bottles, and left to settle, when the clear portion was poured off for use. 



8. The cattle were all inoculated with the virus, as described, by the end of the second 

 day. The virus was perfectly sweet on the second day, and was kept for eight days 

 longer in a useful state, by lilling the bottle to the depth of an inch with neatsfoot 

 oil. 



y. With needle and worsted thread, the thread being put twice through the tail. 

 3 0. From the Sth to the 75th day. Not a single case of excessive swelling took place in 

 the last lot of cattle inoculated, though the effect was visible by the twisting of the 

 tails ; but in the first lot the swellings in al>out one-fourth were more or less excessive, 

 and in many cases the tails dropped off. 



11. None of the cows were milked after inoculation. 



12. From the effects of inoculation, two heifers which were in season when inoculated, died, 

 out of the first lot ; the remainder of those seriously affected were saved by cutting 

 deeply into the swelling and inserting a small piece of rag or two dipped in a mixture 

 of butter of antimony and Venice tui-ps. The two heifers could not be got in in time to 

 dress them. Several of the second lot died after inoculation, but not from excessive 

 sweUing. 



1.3. Satisfactory. 



14. After the first herd were inocvilated they were mixed with the other cattle, and none of 

 them took the disease. In the second herd, many of the cattle which were diseased 

 when inoculated, appeared to be benefited by the operation, the deaths gradually 

 diminishing until March, and there has been no appearance of the disease on the run 

 since. 



1.5. Yes. 



