1911. 



PUBLIC DOCUMENT — No. 31. 



139 



Polarization afler Hydrolysis. 



Invert Sugar 



in 100 Cubic 



Centimeters 



(Crams). 



Saccharimeter 

 Reading. 



Specific 

 Rotatory- 

 Power 

 (Degrees). 



Root 34, 

 Root 40. 

 Syrup A, 

 Syrup B. 

 Syrup C, 

 Extract 1, 

 Extract 2, 



.893 

 1.189 

 1.381 

 1.4C1 

 .452 

 .930 

 2.350 



-2.33 

 -4.10 

 -3.45 

 -5.25 

 -1.30 

 -3.00 

 -7.80 



The action on polarized light both before and after inversion 

 exclndes the possibility of the carbohydrate being pure sucrose, 

 while the failure to secure it with strontium hydrate renders its 

 absence probable. 



Fructose w^as clearly demonstrated by the osazone and the 

 negative optical activity, also by fine reactions with resorcin 

 and hydrochloric acid. Glucose is indicated by the osazone and 

 the fact that the specific rotatory power of the inverted solu- 

 tions is not high enough for pure fructose. Fructose (dearly 

 predominates over the glucose, and the non-reducing property 

 before hydrolysis indicates some condensation product formed 

 between them. The behavior of individual root extracts does 

 not point to any fixed proportion of the two sugars. 



These results are, on the whole, in close agreement with those 

 of Wichers and Tollens. There was, however, a marked differ- 

 ence in the behavior of the water extract of the roots, which con- 

 tained the sugar-like carbohydrate. Wichers and Tollens used 

 boiling water, and state that only a portion of this carbohydrate 

 was soluble in water when extractions were made on the water 

 bath. Their solutions also reduced Fehling's solution before 

 hydrolysis. 



My extractions were all made with water at 20° C, and until 

 hydrolized, had either no reducing action or precipitated no 

 more than traces of copper. 



This diiference in solubility and reducing action is doubtless 



